藏鸡 Chemerin和ChemR23基因时序表达及其与肌内脂肪含量的相关性研究

doi:10.11843/j.issn.0366-6964.2015.08.003

Abstract

Abstract:

This study aimed to clarify the tissue and sequential expression profiles of Chemerin and ChemR23 genes in Tibetan chicken，and to investigate the impacts of the 2 genes expressions on intramuscular fat (IMF) deposition.In this study，Tibetan chicken (0-210 day) was used as experimental animals and the RT-PCR method were employed to amplify the Chemerin and ChemR23 genes.Their relative expression was measured by fluorescent quantitative PCR in various tissues and at different developmental stages.The correlation analysis was performed between gene expression and IMF content.The results indicated that expression of Chemerin and ChemR23 was detected broadly in different tissues.Chemerin was mostly expressed in liver，adipose tissue，lung and kidney of Tibetan chicken， while ChemR23 was expressed in adipose tissue at significantly higher levels than in other tissues (P<0.01).The highest levels were in breast muscle at day 0 and in adipose tissue at day 154，but the expression patterns of the 2 genes in breast muscle and adipose tissue at different developmental stages were not correlated with gender.However，their expression patterns in the thigh muscle were correlated with gender.In female thigh muscle，the relative expression levels of both genes were significantly higher at day 119 than other periods (P<0.01)，but in male thigh muscle，the relative expression level of Chemerin genes were significantly higher at day 0 and 210 than other periods (P<0.01).The peak expression of ChemR23 presented at day 210 in male thigh muscle.The expression of Chemerin and ChemR23 mRNA was positively correlated with IMF content in breast muscle，whereas Chemerin expression was negatively correlated with IMF content in thigh muscle.Interestingly，ChemR23 expression was negatively and positively correlated with IMF content of male and female thigh muscle，respectively.The results suggest that Chemerin and ChemR23 genes may play critical roles in IMF deposition during growth and development of Tibetan chicken，which will help us to understand the biological functions of Chemerin and ChemR23 genes in fat metabolism of Tibetan chicken.

CLC Number:

S831.2

LIN Ya-qiu，XU Ya-ou，ZHANG Run-feng，ZHANG Ming，ZUO Bin，ZHENG Yu-cai. Association Analysis of Sequential Expression of Chemerin and ChemR23 with Intramuscular Fat Contents in Tibetan Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1290-1299.

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Association Analysis of Sequential Expression of Chemerin and ChemR23 with Intramuscular Fat Contents in Tibetan Chicken

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